Multimodal Imaging of Living Cells with Multiplex Coherent Anti-stokes Raman Scattering (CARS), Third-order Sum Frequency Generation (TSFG) and Two-photon Excitation Fluorescence (TPEF) Using a Nanosecond White-light Laser Source - Photonique Fibre et Sources Cohérentes
Article Dans Une Revue Analytical Sciences Année : 2015

Multimodal Imaging of Living Cells with Multiplex Coherent Anti-stokes Raman Scattering (CARS), Third-order Sum Frequency Generation (TSFG) and Two-photon Excitation Fluorescence (TPEF) Using a Nanosecond White-light Laser Source

Hiroki Segawa
  • Fonction : Auteur
Philippe Leproux
Vincent Couderc
Ozawa Takeaki
  • Fonction : Auteur

Résumé

The subnanosecond "white-light laser" source has been applied to multimodal, multiphoton, and multiplex spectroscopic imaging (M(3) spectroscopic imaging) with coherent anti-Stokes Raman scattering (CARS), third-order sum frequency generation (TSFG), and two-photon excitation fluorescence (TPEF). As the proof-of-principle experiment, we performed simultaneous imaging of polystyrene beads with TSFG and TPEF. This technique is then applied to live cell imaging. Mouse L929 fibroblastic cells are clearly visualized by CARS, TSFG, and TPEF processes. M(3) spectroscopic imaging provides various and unique cellular information with different image contrast based on each multiphoton process.
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Dates et versions

hal-01321491 , version 1 (01-10-2024)

Identifiants

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Hiroki Segawa, Masanari Okuno, Philippe Leproux, Vincent Couderc, Ozawa Takeaki, et al.. Multimodal Imaging of Living Cells with Multiplex Coherent Anti-stokes Raman Scattering (CARS), Third-order Sum Frequency Generation (TSFG) and Two-photon Excitation Fluorescence (TPEF) Using a Nanosecond White-light Laser Source. Analytical Sciences, 2015, 31, pp.299-305. ⟨10.2116/analsci.31.299⟩. ⟨hal-01321491⟩
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