Hyalomma scupense (Acari, Ixodidae) is a common tick species found in several areas in North Africa, Asia and South Europe and an efficient natural vector of bovine tropical theileriosis (Theileria annulata), a livestock disease with an important economic impact. For one year, 1938 ticks were collected on cattle in several Corsican slaughterhouses; 168 of them were morphologically identified as H. scupense. This result was confirmed by genetic identification using sequences of mitochondrial cytochrome c oxidase subunit I (COI) and ribosomal internal transcribed spacer 2 (ITS2) genes. The presence of 2 different stages (adults and nymphs), collected in various areas of the island, indicates that a population of H. scupense is established in Corsica. However, bovine tropical theileriosis has not been diagnosed on the island so far.